I suggest that you use the InputStream property of the UploadedFile object that is available in the FileUploaded event. It is a stream pointing to the uploaded file, and you can use it in order to read the file.
sender, FileUploadedEventArgs e)
 buffer =
(Stream str = e.File.InputStream)
str.Read(buffer, 0, e.File.ContentLength);
// use the read bytes
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