RadScriptManager is trying to load WebUIValidation from an address that does not exist.
I have two scenarios:
1 - When the RadScriptManager is configure like below [0] the script tag [1] is generated which try to load the script from an unexistent place.
[0] -
<telerik:RadScriptManager ID="RadScriptManager1" runat="server" AsyncPostBackTimeout="500"
ScriptPath="~" ScriptMode="Release">
<Scripts>
.... custom scripts ...
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.Core.js" />
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.jQuery.js" />
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.jQueryInclude.js" />
</Scripts>
</telerik:RadScriptManager>
[1] -
<script src="System.Web/4.0.0.0/4.6.1637.0/WebUIValidation.js" type="text/javascript"></script>
2 - When the RadScriptManager is configure like below [2] there is no error to load the script, however the error happens when a content is loaded via Ajax using the RadAjaxManagerProxy at this time it's triggered by an internal call of Telerik.Web.UI.WebResource.axd.
<telerik:RadScriptManager ID="RadScriptManager1" runat="server" AsyncPostBackTimeout="500"
EnablePageMethods="true" ScriptPath="~" ScriptMode="Release" >
<Scripts>
.... custom scripts ...
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.Core.js" />
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.jQuery.js" />
<asp:ScriptReference Assembly="Telerik.Web.UI" Name="Telerik.Web.UI.Common.jQueryInclude.js" />
<asp:ScriptReference Assembly="System.Web, Version=4.0.0.0, Culture=neutral, PublicKeyToken=b03f5f7f11d50a3a" Path="~/js/WebUIValidation.js" Name="WebUIValidation.js" />
</Scripts>
</telerik:RadScriptManager>