How can I get specific row's column value of GridDataItem (vb.net) ?

[Matthew]

There is the a form:

|    Segment   |        TimeIn       |       TimeOut        |       Duration      |

|          1          |   XX:XX:XX:XX  |   XX:XX:XX:XX   |   XX:XX:XX:XX  |

|          2         |   XX:XX:XX:XX  |   XX:XX:XX:XX   |   XX:XX:XX:XX  |

|          3         |   XX:XX:XX:XX  |   XX:XX:XX:XX   |   XX:XX:XX:XX  |

|          4         |   XX:XX:XX:XX  |   XX:XX:XX:XX   |   XX:XX:XX:XX  |

The number of Segment depends on how many xml file is found. I want to limited the information cannot be empty in middle (without the 1st seg1 and last seg4). How can I set get these middle segment number? My way is using  SegmentNo.Text <> 1 and SegmentNo.Text <> last. However it can't use ".last". How can I get the last row's Segment No? 

Code

For Each r As GridDataItem In gridSegment.Items

   If FileName.Text <> "" Then

       // Red the textbox if data is missing

   Else

      If timeIn.Text = "" AND timeOut.Text = "" AND Duration.Text = "" Then

        If Segment.Text <> 1 AND Segment.Text <> ??? Then
        
          // Red that row all textbox

        End If

     End If

   End If

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