You can define the desired style for the GridViewCell of the column you are creating programatically and set it as a its CellStyle. For example:
And then in the code-behind:
Private Sub AddColumn(sender As Object, e As RoutedEventArgs)
Dim newColumn As New GridViewDataColumn()
newColumn.DataMemberBinding = New Binding("Name")
newColumn.CellStyle = DirectCast(Me.Resources("NewColumnStyle"), Style)
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